AP BIOLOGY

Detailed explanation-1: -The principle of recombinant DNA technology involved four steps. The four steps are: (1) Gene Cloning and Development of Recombinant DNA (2) Transfer of Vector into the Host (3) Selection of Transformed Cells and (4) Transcription and Translation of Inserted Gene.

Detailed explanation-2: -Following steps are involved in the recombinant DNA technology: DNA containing the gene of interest is identified and isolated from the organism. The gene of interest is inserted into the plasmid which is a vector which is used to deliver and maintain the DNA in the host organism.