THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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LacZ gene
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Ampicillin resistant gene
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LacY gene
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LacA gene
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Detailed explanation-1: -If the foreign DNA is not inserted into the vector or if it is inserted at a location other than MCS, the lacZ gene in the plasmid vector complements the lacZ deletion mutation in the host E. coli producing a functional enzyme.
Detailed explanation-2: -LacZ gene is present in the plasmid vectors . They possess a short sequence in the these cvectors . These genes are essential as they contain 146 amino acid codes of the enzyme beta-galactosidase. This enzyme is useful for the metabolism of latose .
Detailed explanation-3: -Inserting genes into plasmids The piece of DNA or gene of interest is cut from its original DNA source using a restriction enzyme and then pasted into the plasmid by ligation. The plasmid containing the foreign DNA is now ready to be inserted into bacteria. This process is called transformation.
Detailed explanation-4: -What is the purpose of the LacZ gene in a plasmid cloning vector? It is used as a selectable marker. The gene contains a series of unique restriction sites where a fragment of DNA can be inserted and cloned.