THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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So that scientists can make antibiotic resistant bacteria and make more money for pharmaceutical companies.
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So that scientists can create super bugs.
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So that scientists can identify which cells have the rDNA plasmid.
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So that scientists can kill the bacteria.
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Detailed explanation-1: -Adding an antibiotic resistance gene to the plasmid solves both problems at once – it allows a scientist to easily detect plasmid-containing bacteria when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid.
Detailed explanation-2: -Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony. Colonies with the right plasmid can be grown to make large cultures of identical bacteria, which are used to produce plasmid or make protein.
Detailed explanation-3: -A plasmid typically contains an antibiotic resistance gene, which allows bacteria to survive in the presence of a specific antibiotic. Thus, bacteria that took up the plasmid can be selected on nutrient plates containing the antibiotic.
Detailed explanation-4: -Antibiotics commonly used for plasmid selection These antibiotic resistance genes not only give the scientist with an easy way to detect plasmid-containing bacteria, but also provide those bacteria with a pressure to maintain and replicate your plasmid over multiple generations.