THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
plasmid
|
|
|
restriction enzyme
|
|
|
ligase
|
|
|
protein expression
|
Detailed explanation-1: -The enzyme DNA ligase joins the fragments with matching ends together to form a single, unbroken molecule of DNA.
Detailed explanation-2: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-3: -Ligase is an enzyme that is able to join two different fragments of DNA together. The sticky ends of the DNA fragments are complementary to each other, allowing ligase to bind them together, sealing the gene into the plasmid.
Detailed explanation-4: -DNA Ligase joins DNA fragments.
Detailed explanation-5: -DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond.