AP BIOLOGY

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THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of the following is correct sequence of process in production of cDNA?Reverse transcriptase used to synthesis single stranded cDNA complementary to mRNARemove mRNAExtract mRNA from cellUse DNA polymerase to synthesis second stranded cDNA
A
1, 2, 3, 4
B
3, 4, 2, 1
C
3, 1, 2, 4
D
2, 3, 1, 4
Explanation: 

Detailed explanation-1: -cDNA is DNA that is complementary to the mRNA transcribed from the gene. Because mRNA is processed, it will lack introns and regulatory elements. cDNA cloning can produce a cDNA library or produce probes for screening genomic libraries.

Detailed explanation-2: -Reverse transcription reactions involve three main steps: primer annealing, DNA polymerization, and enzyme deactivation. The temperature and duration of these steps vary by primer choice, target RNA, and reverse transcriptase used.

Detailed explanation-3: -First-strand synthesis of cDNA utilizes either oligo(dT), random primers, or a combination of these strategies to prime the reverse transcription reaction. Priming a reaction with oligo(dT) initiates the synthesis preferentially at the 3’ end of the RNA fragment.

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