THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
they must be from the same species
|
|
|
their strands must separate as in replication
|
|
|
must be cut and spliced at specific nucleotide sequences
|
|
|
undergo lysis
|
Detailed explanation-1: -Recombinant DNA technology Certain enzymes called restriction enzymes are used in laboratories to splice, connect (or ligate), and remove or add nucleotides to sequences. Restriction enzymes are used in recombinant DNA technology to remove and insert genetic sequences from and into other sequences.
Detailed explanation-2: -Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragments at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends.
Detailed explanation-3: -Ligation of DNA Molecules The purified DNA and the vector of interest are cut with the same restriction enzyme. This gives us the cut fragment of DNA and the cut vector, that is now open. The process of joining these two pieces together using the enzyme ‘DNA ligase’ is ‘ligation’.