AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Which of the following is NOT a tool used in recombinant DNA technology?
A
Cloning vector
B
Restriction endonuclease
C
DNA polymerase
D
DNA ligase
Explanation: 

Detailed explanation-1: -So, the correct option is ‘Introns’.

Detailed explanation-2: -RT is used to synthesize complementary strand (cDNA) from mRNA template. It is also known as RNA dependent DNA polymerase.

Detailed explanation-3: -Recombinant DNA technology can be accomplished only if we have the following key tools i.e., reaction enzymes, polymerase enzyme, ligases and vectors.

Detailed explanation-4: –The type-I recognizes specific sequences in the DNA of interest but at non-specific sites. It cuts at more than 1, 000 bp away from the gene of interest. Thus, it is incompatible to be used.

There is 1 question to complete.