THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which ONE is not used in recombinant DNA technology?
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Cloning vector
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RNA polymerase
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Target DNA
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DNA ligase
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Explanation:
Detailed explanation-1: -So, the correct option is ‘Introns’.
Detailed explanation-2: -RT is used to synthesize complementary strand (cDNA) from mRNA template. It is also known as RNA dependent DNA polymerase.
Detailed explanation-3: –The type-I recognizes specific sequences in the DNA of interest but at non-specific sites. It cuts at more than 1, 000 bp away from the gene of interest. Thus, it is incompatible to be used.
Detailed explanation-4: -So, the correct option is ‘DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments’.
Detailed explanation-5: -Recombinant DNA technology can be accomplished only if we have the following key tools i.e., reaction enzymes, polymerase enzyme, ligases and vectors.
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