THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Antibiotics will prevent the growth of bacteria without plasmid
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Bacteria cells with recombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal
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Bacteria cells which contain non-recombinant plasmids are resistant to antibiotics and unable to hydrolyse X-gal
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Detailed explanation-1: -For screening the clones containing recombinant DNA, a chromogenic substrate known as X-gal is added to the agar plate. If -galactosidase is produced, X-gal is hydrolyzed to form 5-bromo-4-chloro-indoxyl, which spontaneously dimerizes to produce an insoluble blue pigment called 5, 5’-dibromo-4, 4’-dichloro-indigo.
Detailed explanation-2: -1. Which of the following is true regarding taking up of plasmid DNA in the bacterial cells? Explanation: There are more chances of having a single plasmid in one cell.
Detailed explanation-3: -Once a recombinant plasmid is made that contains a gene of interest, such as insulin, the plasmid can enter bacterial cells by a process called transformation.
Detailed explanation-4: -We should use cDNA cloning. In this technique, we isolate mRNA from the cytoplasm of a cell, so that it has already been spliced. We then use reverse transcriptase enzyme to make a DNA copy (cDNA) of the mRNA. This DNA can then be cloned, producing a bacteria-readable gene that lacks introns.