THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The cell that prevent plasmid from multiplying
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The cell that unable to receive cDNA through transformation
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Plasmid is an example of host cell
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The cell that able to receive recombinant DNA for cloning purpose
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Detailed explanation-1: -Host organism – into which the recombinant DNA is introduced. The host is the ultimate tool of recombinant DNA technology which takes in the vector engineered with the desired DNA with the help of the enzymes.
Detailed explanation-2: -In practice the procedure is carried out by inserting a DNA fragment into a small DNA molecule and then allowing this molecule to replicate inside a simple living cell such as a bacterium. The small replicating molecule is called a DNA vector (carrier).
Detailed explanation-3: -Today, the E. coli bacteriophage is one of the most widely used vectors used to carry recombinant DNA into bacterial cells. This virus makes an excellent vector because about one-third of its genome is considered nonessential, meaning that it can be removed and replaced by foreign DNA (i.e., the DNA being inserted).
Detailed explanation-4: -Recombinant DNA technology requires both nucleases and ligases. II. Ligases produce nicks in the recombinant DNA molecule.