THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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It cuts the DNA to obtain the gene of interest.
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It breaks the DNA molecule to produce multiple fragments.
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It stimulates the replication of a recombinant DNA.
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It seals the inserted gene of interest to an open plasmid.
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Detailed explanation-1: -(Science: enzyme molecular biology) An enzyme that introduces minor bases into dNA or rNA or that alters bases already incorporated. Serves to alter the sequence so that restriction enzymes will not damage the strand.
Detailed explanation-2: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-3: -The plasmid is cut with the same restriction enzymes so it gets the same sticky ends. The sticky ends on the plasmid stick with the ones on the gene. The gene and the plasmid are joined together using an enzyme called DNA ligase . The vector is transferred back into the bacteria host cells.
Detailed explanation-4: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.