THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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restriction endonuclease-production of DNA fragments for gene cloning.
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DNA ligase-enzyme that cuts DNA, creating sticky ends.
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reverse transcriptase-production of cDNA from mRNA.
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Taq polymerase-copies DNA sequences in the polymerase chain reaction.
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Detailed explanation-1: -Answer and Explanation: The correct answer is (b) DNA ligase cutting DNA, creating sticky ends of restriction fragments. DNA ligase is an enzyme that connects the sugar-phosphate backbone of adjacent nucleotides, combining two separate strands of DNA into a single sequence.
Detailed explanation-2: -So, the correct option is ‘Introns’.
Detailed explanation-3: -Solution : ‘DNA‘ ligases are also called genetic gum. They join two indiviual fragments of double stranded DNA by forming phosphodiester bonds between them thus help in sealing of DNA fragments. Therefore acts as molecular glue.
Detailed explanation-4: -They shorten the DNA by one nucleotide at a time, they do not digest it into fragments. Thus the correct answer is option D.