AP BIOLOGY

Detailed explanation-1: -Restriction endonucleases are molecular scissors that recognize short DNA sequences usually 4–8 bp in length and cut the phosphodiester bond within or close to their target sites to generate a double-stranded break.

Detailed explanation-2: -Introduction. Restriction enzymes are also called “molecular scissors” as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.

Detailed explanation-3: -Restriction Enzyme A restriction enzyme is a protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end.

Detailed explanation-4: -These enzymes are called restriction enzymes, or restriction endonucleases. Restriction enzymes are proteins produced by bacteria to prevent or restrict invasion by foreign DNA. They act as DNA scissors, cutting the foreign DNA into pieces so that it cannot function.

Detailed explanation-5: -Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs.