AP BIOLOGY

Detailed explanation-1: -Some restriction enzymes leave behind nucleotide tails, or “sticky ends, ” when they cut DNA.

Detailed explanation-2: -Some restriction enzymes restriction enzymes leave behind nucleotide tails, or “sticky ends, ” when they cut DNA. Use virtual restriction enzymes at ClassZone.com.

Detailed explanation-3: -Restriction enzymes hydrolyze covalent phosphodiester bonds of the DNA to leave either “sticky/cohesive” ends or “blunt” ends. This distinction in cutting is important because an EcoRI sticky end can be used to match up a piece of DNA cut with the same enzyme in order to glue or ligate them back together.

Detailed explanation-4: -Mertz and Davis discovered that another restriction enzyme, EcoR1, by contrast, cleaves its recognition site in a staggered way that generates fragments with single-stranded overhanging ends known as cohesive, or sticky, ends.

Detailed explanation-5: -A ‘sticky’ end is produced when the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, then cuts on the opposite end of the complementary strand. This will produce two ends of DNA that will have some nucleotides without any complementary bases.