AP BIOLOGY

Detailed explanation-1: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.

Detailed explanation-2: -They recognize and bind to specific sequences of DNA, called restriction sites. Each restriction enzyme recognizes just one or a few restriction sites. When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.

Detailed explanation-3: -Restriction Enzyme Recognition Site Many restriction enzymes or restriction endonucleases identify a specific palindrome site and cut the DNA strands. They mostly cut the DNA, slightly away from the centre of the palindrome and between the same two nitrogenous bases in both the strands.

Detailed explanation-4: -Restriction enzymes, also called restriction endonucleases, cut double stranded DNA molecules by cleaving phosphodiester bonds at palindromic sequences. This means that the majority of the restriction enzymes cut the DNA into a fragments that are characterized by a twofold rotational symmetry.

Detailed explanation-5: -Restriction enzymes cut double-stranded DNA * at specific locations based the pattern of bases found at those locations. These enzymes predictably cut both strands because the sequences they recognize are palindromic. That is the recognition sequences are short string of identical bases on both DNA strands.