THE MOLECULAR BASIS OF INHERITANCE
TOOLS AND TECHNIQUES OF RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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They are named according to their actions.
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They cut double-stranded DNA to produce blunt ends.
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They cut double-stranded DNA at specific nucleotide sequences.
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They are used in recombinant DNA technology to join two DNA fragments.
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Detailed explanation-1: -So, the correct answer is ‘The enzyme binds DNA at specific sites and cuts only one of the two strands’
Detailed explanation-2: -Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location. They are indispensable to the isolation of genes and the construction of cloned DNA molecules.
Detailed explanation-3: -Which statement best describes restriction enzymes? They are important for cloning applications because they can be used to cut DNA at specific nucleotide sequences.
Detailed explanation-4: -Restriction endonucleases (REs) are bacterial enzymes that cleave double-stranded DNA. Type I REs are important in bacterial function but do not cleave DNA at specific sequences.