FOOD MICROBIOLOGY
MICROORGANISMS IN FOOD
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Range of colonies required for counting under PCA agar for estimation of microorganisms in CFU/ml?
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20-150 colonies
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20-200 colonies
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20-300 colonies
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20-400 colonies
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Explanation:
Detailed explanation-1: -The suitable colony counting range (10) is 25-250. The automated spiral plate count method for the examination of foods and cosmetics (5), outlined below, conforms to AOAC Official Methods of Analysis, sec. 977.27.
Detailed explanation-2: -The plate count is linear for E. coli over the range of 30 to 300 CFU on a standard sized Petri dish. Therefore, to ensure that a sample will yield CFU in this range requires dilution of the sample and plating of several dilutions.
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