FOOD MICROBIOLOGY
MICROORGANISMS IN FOOD
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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1 ml
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0.5 ml
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0.2 ml
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0.1 ml
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Detailed explanation-1: -Sample volume to be plated should be between 0.1 and 0.2 ml.
Detailed explanation-2: -The sample must be 0.1 mL (0 to 1 mL is the permitted range) for spreading. If the sample is 1 mL or more, then there will be fused colonies, and also the sample may not be absorbed by the media leaving it to float on the surface. Media should be properly solidified before use.
Detailed explanation-3: -A convenient inoculum volume, in terms of spreading, absorption, and calculations, is 0.1 ml (100 microliters). Since some bacteria rapidly attach to the agar surface, the inoculum should be spread soon after it is applied.
Detailed explanation-4: -1:10 dilution is performed by placing 1 ml of the re-hydrated pellet solution into 9 ml of buffer = 100-1, 000 CFU/ml. 3. 0.1 ml of the organism suspension plated to an agar = 10-100 CFU per 0.1 ml.