FOOD MICROBIOLOGY
MICROORGANISMS IN FOOD
Question
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Lactose Sulphate Trypsin Broth
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Lauryl Sulphate Tryptose Broth
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Lectin Sulphate Tryptose Broth
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Lauryl Sucrose Trypsin Broth
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Detailed explanation-1: -Remel Lauryl Tryptose Broth (LST Broth) (Lauryl Sulfate Tryptose) is a liquid medium recommended for use in qualitative procedures for the detection of coliforms in water, wastewater, and foods.
Detailed explanation-2: -Principle and Interpretation: Sodium lauryl sulphate inhibits organisms other than coliforms. Bile salts inhibit gram-positive bacteria especially bacilli and faecal Streptococci. Sodium chloride maintains the osmotic balance of the medium.
Detailed explanation-3: -Lauryl Sulfate Broth is a selective growth medium used for the detection of coliform bacteria in water and wastewater in a laboratory setting. The coliform group includes aerobic and facultative anaerobic, Gram-negative and non-spore-forming bacilli, which ferment lactose and form acid and gas at 35°C in 48 hours.