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Detailed explanation-1: -The first step in gram staining is the use of crystal violet dye for the slide’s initial staining. The next step, also known as fixing the dye, involves using iodine to form crystal violet-iodine complex to prevent easy removal of dye.

Detailed explanation-2: -A Gram stain is colored purple. When the stain combines with bacteria in a sample, the bacteria will either stay purple or turn pink or red. If the bacteria stays purple, they are Gram-positive. If the bacteria turns pink or red, they are Gram-negative.

Detailed explanation-3: -The gram stain is the most frequently used stain in a clinical microbiology laboratory and is usually the first step in identifying bacteria. Based on differences in cell wall components, bacteria are categorized as either gram-positive (stains dark purple) or gram-negative (stains pink).