FOOD TECHNOLOGY

Detailed explanation-1: -During the denaturation of proteins, the secondary and tertiary structures get destroyed and only the primary structure is retained. Covalent bonds are broken and interaction between amino-acid chains gets disrupted. This results in the loss of biological activity of the proteins.

Detailed explanation-2: -Precipitation occurs because the change in pH or hydrophobicity alters interactions between the protein and the aqueous environment or through binding of salts or metals to protein functional groups such that intramolecular interactions are disrupted and the proteins denature, aggregate, and fall out of solution [5].

Detailed explanation-3: -In secondary structure denaturation, hydrogen bonds are disturbed. Proteins lose the regular repeating patterns such as -helix and -sheets, and adopt a random coil configuration. Primary structure is held by covalent peptides, which are not disrupted by denaturation.