AP BIOLOGY

Detailed explanation-1: -Non-conservative mutations can be more severe because they cause a greater change to the protein sequence or expression level. An example would be changing an acidic amino acid into a basic one.

Detailed explanation-2: -Mutations caused by insertion or deletion of nitrogenous bases in the DNA sequence leads to the most damage to DNA since it completely changes the reading frame for the process of protein synthesis. Insertion or deletion that results in frameshift mutation are considered as the most damaging mutation.

Detailed explanation-3: -Frameshift mutations are generally much more serious and often more deadly than point mutations. Even though only a single nitrogen base is affected, as with point mutations, in this instance, the single base is either completely deleted or an extra one is inserted into the middle of the DNA sequence.