AP BIOLOGY

Detailed explanation-1: -DNA polymerase I removes the RNA primer and fills in the gaps with DNA. However, DNA polymerase I cannot catalyze the reaction to remove the nicks. Another enzyme, DNA ligase, seals the nicks by forming the phosphodiester bond, thus generating a continuous sugar-phosphate backbone for the lagging strand.

Detailed explanation-2: -Because of its 5′ to 3′ exonuclease activity, DNA polymerase I removes RNA primers and fills the gaps between Okazaki fragments with DNA.

Detailed explanation-3: -RNA primers are removed and replaced with DNA by DNA polymerase I. The gaps between DNA fragments are sealed by DNA ligase.

Detailed explanation-4: -DNA polymerase I is recognized as an enzyme that fills any remaining gaps between Okazaki fragments and removes RNA primers with a duplex-specific 5′-3′ exonuclease activity, providing a nick that can be sealed by DNA ligase 31, 32.

Detailed explanation-5: -DNA polymerase I (pol I) processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions.