THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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recombinant, plasmid, hydrogen
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cloning, ribosome, hydrogen
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target, vector, phosphodiester
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complementary, mRNA, phosphodiester
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Detailed explanation-1: -Foreign DNA is inserted into a plasmid (or any cloning vector) by ligating the DNA into a complementary site in the plasmid. These sites are generated by digesting the DNA and vector with the same restriction enzyme. (The site for the restriction enzyme that is chosen should only be represented once in the plasmid.
Detailed explanation-2: -Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid.
Detailed explanation-3: -DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.
Detailed explanation-4: -18.1 Introduction. Genetic engineering (or recombinant DNA technology) allows selected individual gene sequences to be transferred from an organism into another and also between nonrelated species.