AP BIOLOGY

Detailed explanation-1: -Recombinant DNA technology is the joining together of DNA molecules from two different species. The recombined DNA molecule is inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry.

Detailed explanation-2: -Plasmids with foreign DNA inserted into them are called recombinant DNA molecules because they contain new combinations of genetic material. Proteins that are produced from recombinant DNA molecules are called recombinant proteins. Not all recombinant plasmids are capable of expressing genes.

Detailed explanation-3: -Indirect gene transfer: In this method, a vector is used to insert the foreign DNA into the host cell.

Detailed explanation-4: -The enzyme DNA ligase joins the fragments with matching ends together to form a single, unbroken molecule of DNA. This produces a recombinant plasmid that contains the target gene.

Detailed explanation-5: -In recombinant technologies, the desired gene selected which is followed by selecting a perfect vector into which the desired gene has to be integrated and then recombinant DNA is formed by ligating the gene of interest with the vector.