THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To identify the particular region of DNA to be copied by PCR.
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To create DNA nucleotides
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To copy DNA.
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To maintain the temperature of the PCR reaction.
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Detailed explanation-1: -In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified, resulting in millions and millions of copies in a very short timeframe. Primers are also used in DNA sequencing and other experimental processes.
Detailed explanation-2: -The synthesis of a primer is necessary because the enzymes that synthesize DNA, which are called DNA polymerases, can only attach new DNA nucleotides to an existing strand of nucleotides. The primer therefore serves to prime and lay a foundation for DNA synthesis.
Detailed explanation-3: -Primers allow DNA polymerase to start elongating DNA. Primers are used in the Polymerase Chain Reaction (PCR) as a step in amplifying a short sequence of DNA. They are unique as they only bind to particular region in DNA where DNA transcription starts off from 5’ to 3’.
Detailed explanation-4: -PCR primers are short, single-stranded segments of DNA that are designed to be complementary to the beginning and end of the target sequence that will be amplified. In a PCR, it is the primers that dictate exactly what sequence of DNA gets copied.
Detailed explanation-5: -Crucial for the overall success of a PCR experiment is the careful design of synthetic oligonucleotide primers. Ideally designed primer pairs will ensure the efficiency and specificity of the amplification reaction, resulting in a high yield of the desired amplicon.