AP BIOLOGY

Detailed explanation-1: -Alkaline phosphatase (ALP) (EC 3.1. 3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation.

Detailed explanation-2: -Alkaline phosphatase is used to remove phosphate at the 5’ end.

Detailed explanation-3: -Alkaline phosphatase (ALP) is a hydrolase that removes the phosphate group from various proteins and nucleotides.

Detailed explanation-4: -Although there are a variety of sources for these enzymes, the most common are calf intestinal alkaline phosphatase (CIAP) and T4 polynucleotide kinase (T4 PNK). Their most frequent use is to modify the phosphorylation state of the 5′-ends of DNA molecules (Figure 1, Panels D-H).

Detailed explanation-5: -Alkaline phosphatase is the enzyme that catalyzes a dephosphorylation of DNA, RNA, ribo-, and deoxyribonucleoside triphosphates. It can also remove phosphates from nucleotides and proteins. They are most active at a basic pH.