AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
Restriction enzymes cleave DNA by cutting the
A
hydrogen bond between the nitrogenous base pair
B
phosphodiester bond between the deoxyribose sugar and phosphate group
C
glycosidic bond between the deoxyribose sugar and nitrogenous base
D
covalent bond in the nitrogenous base
Explanation: 

Detailed explanation-1: -Restriction enzymes break phosphodiester bonds between the phosphate and the pentose sugar in sugar-phosphate backbone at the specific site.

Detailed explanation-2: -Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo, DNases or RNases, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes.

Detailed explanation-3: -DNA ligases are enzymes that can form a phosphodiester bond at a single-strand break in DNA, a reaction between a 3′-OH group and a 5′-monophosphate.

Detailed explanation-4: -Restriction enzymes cleave the phosphodiester bond between the phosphate and the pentose sugar at a particular location in the sugar-phosphate backbone.

There is 1 question to complete.