Which of the following is TRUE regarding screening by using antibiotics and X-gal? I. Antibiotics will prevent the growth of bacteria without plasmid. II Bacteria cells with recombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal. III. Bacteria cells which contain nonrecombinant plasmids are resistant to antibiotics and are unable to hydrolyse X-gal.

RECOMBINANT DNA

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Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]

A	I only.
B	I and II only.
C	II and III only.
D	All of the above.

Explanation:

Detailed explanation-1: -A classic method for detecting the presence of antibiotics is the use of microbiological assays that employ antibiotic-sensitive species of bacteria to determine whether specific antibiotics are present in a given sample and, with limited sensitivity, their concentration.

Detailed explanation-2: -The vast majority of the screening methods used for monitoring the presence of antimicrobial compounds today are still microbial inhibition tests [17].

Detailed explanation-3: -Which of the following is/are true of antibiotic usage and the development of antibiotic resistance? Antibiotics increase the rate of bacterial transformation, conjugation, and transduction, and thus increase the spread of antibiotic resistance.

There is 1 question to complete.

AP BIOLOGY

THE MOLECULAR BASIS OF INHERITANCE

RECOMBINANT DNA