THE MOLECULAR BASIS OF INHERITANCE
RECOMBINANT DNA
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?I) Transform bacteria with recombinant DNA molecule.II) Cut the phosphodiester bond of both DNAs using restriction enzymes.III) Extract plasmid DNA from bacterial cells.IV) Hydrogen bond formed between the plasmid and DNA fragments.V) Use ligase to seal plasmid and target gene
|
|
I, II, IV, III, V
|
|
|
III, II, IV, V, I
|
|
|
II, III, V, IV, I
|
|
|
III, IV, V, I, II
|
Explanation:
Detailed explanation-1: -(i) Transformbacteria with recombinant DNA molecule. (ii) Cut the plasmid DNA using restriction enzymes. (iii) Extract plasmid DNA from bacterial cells. (iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
Detailed explanation-2: -The correct option is B I
There is 1 question to complete.