FOOD MICROBIOLOGY
MICROORGANISMS IN FOOD
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which agar selectively for detecting vibrio spp?
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EMB agar
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Mac Conkey agar
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TCBS agar
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Salmonella Shigella Agar
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Explanation:
Detailed explanation-1: -Alkaline peptone water (APW) is recommended as an enrichment broth, and thiosulfate citrate bile salts sucrose (TCBS) agar is the selective agar medium of choice for isolating V.
Detailed explanation-2: -In BD TCBS Agar, yeast extract and peptone provide the nitrogen and vitamins. Sodium citrate, sodium thiosulfate, oxgall, and cholate are selective agents which provide an alkaline pH to inhibit gram-positive organisms and suppress coliforms.
Detailed explanation-3: -The thiosulfate-citrate-bile salts-sucrose agar (TCBS) used in the most-probable-number method for detecting Vibrio parahaemolyticus cannot differentiate growth of V. parahaemolyticus from Vibrio vulnificus or Vibrio mimicus.
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