FOOD TECHNOLOGY

SUSTAINABILITY IN FOOD TECHNOLOGY

SUSTAINABLE FOOD PACKAGING

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
The total plate count of a milk sample is 2.34×106 CFU/mL. What final dilution factor would yield a countable plate?(Countable range:30-300 colonies; volume plated:1 mL)
A
104
B
10-4
C
105
D
10-5
E
10-4 and 10-5
Explanation: 

Detailed explanation-1: -If there is one colony on the plate and 1ml was plated, the result is 10 CFU/ml. If 100ul were plated the result would be 100 CFU/ml. If there are five colonies on the plate, the count is 50 CFU (or 50 CFU/ml if 1ml is plated). Because there can’t be between 0 and 1 colony, the minimum countable result is 10 cfu.

Detailed explanation-2: -CFU/100 mL = (# of colonies counted ÷ sample volume filtered in mL) x 100 (CFU = colony forming units).

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